Plasmid

Part:BBa_K3258009

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


Selection Plasmid PREDCEL+

This plagemid encodes the M13 genes III and IV along with a mRFP. Through NdeI digestion, the backbone can be opened up and mRFP replaced with the gene the user wants to evolve with PREDCEL+.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
    Illegal EcoRI site found at 2403
    Illegal EcoRI site found at 2533
    Illegal PstI site found at 4802
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
    Illegal EcoRI site found at 2403
    Illegal EcoRI site found at 2533
    Illegal NheI site found at 26
    Illegal NheI site found at 49
    Illegal PstI site found at 4802
    Illegal NotI site found at 3492
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
    Illegal EcoRI site found at 2403
    Illegal EcoRI site found at 2533
    Illegal BglII site found at 8756
    Illegal BamHI site found at 8
    Illegal XhoI site found at 1810
    Illegal XhoI site found at 3501
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
    Illegal EcoRI site found at 2403
    Illegal EcoRI site found at 2533
    Illegal PstI site found at 4802
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
    Illegal EcoRI site found at 2403
    Illegal EcoRI site found at 2533
    Illegal PstI site found at 4802
    Illegal NgoMIV site found at 2086
    Illegal NgoMIV site found at 3800
    Illegal NgoMIV site found at 6976
    Illegal NgoMIV site found at 7136
    Illegal NgoMIV site found at 8724
    Illegal AgeI site found at 3233
    Illegal AgeI site found at 3345
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None