Plasmid
Part:BBa_K3258009
Designed by: Alec Lourenco Group: iGEM19_Stanford (2019-10-16)
Selection Plasmid PREDCEL+
This plagemid encodes the M13 genes III and IV along with a mRFP. Through NdeI digestion, the backbone can be opened up and mRFP replaced with the gene the user wants to evolve with PREDCEL+.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 14
Illegal EcoRI site found at 2403
Illegal EcoRI site found at 2533
Illegal PstI site found at 4802 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 14
Illegal EcoRI site found at 2403
Illegal EcoRI site found at 2533
Illegal NheI site found at 26
Illegal NheI site found at 49
Illegal PstI site found at 4802
Illegal NotI site found at 3492 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 14
Illegal EcoRI site found at 2403
Illegal EcoRI site found at 2533
Illegal BglII site found at 8756
Illegal BamHI site found at 8
Illegal XhoI site found at 1810
Illegal XhoI site found at 3501 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 14
Illegal EcoRI site found at 2403
Illegal EcoRI site found at 2533
Illegal PstI site found at 4802 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 14
Illegal EcoRI site found at 2403
Illegal EcoRI site found at 2533
Illegal PstI site found at 4802
Illegal NgoMIV site found at 2086
Illegal NgoMIV site found at 3800
Illegal NgoMIV site found at 6976
Illegal NgoMIV site found at 7136
Illegal NgoMIV site found at 8724
Illegal AgeI site found at 3233
Illegal AgeI site found at 3345 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |